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KMID : 1123920080220020422
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Korean Journal of Oriental Physiology and Pathology
2008 Volume.22 No. 2 p.422 ~ p.426
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Protective Effect of Radix Trichosanthis Extracts on Cytotoxicity of Pancreatic by Cytokines
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Song Mi-Young
Kim Eun-Kyung
Song Je-Ho
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Abstract
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In this study, the preventive effects of Radix Trichosanthis extracts (RTE) against cytokine-induced death were assessed. Cytokines generated by immune cells infiltrating pancreatic islets are crucial mediators of destruction in insulin-dependent diabetes mellitus. The treatment of RIN cells with () and () resulted in a reduction of cell viability. RTE protected and -mediated viability reduction in a concentration-dependent manner. Incubation with RTE also induced a significant suppression of and -induced inducible nitric oxide synthase (iNOS) protein expression. The molecular mechanism by which RTE inhibited iNOS protein expression appeared to involve the inhibition of activation. The and -stimulated RIN cells showed increases in binding activityand degradation in cytosol compared to unstimulated cells. However, pretreatment with RTE inhibited cytokines-induced degradation and activation in RINm5F cells. Furthermore, the protective effects of RTE were verified via protection of impairment in glucose-stimulated insulin secretions in and -treated islets.
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KEYWORD
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Radix Trichosanthis, cytokine
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